![]() ![]() The timing of this histone release from DNA correlates well with the progression of apoptosis. We report here that in response to apoptotic signals from a death receptor (CD95 and tumor necrosis factor-alpha) or mitochondrial (staurosporine) apoptotic stimulus, the core nucleosomal histones H2A, H2B, H3, and H4 become separated from DNA during apoptosis in Jurkat and HeLa cells and are consequently detectable in the cell lysate prepared using a non-ionic detergent. It is highly probable that nucleosomal structure must be altered to allow these features to become apparent, but data to support this construct are lacking. Chromatin condensation and DNA fragmentation are the classic nuclear features but remain poorly characterized. Chromatin structure is influenced by histone modification, and this may help direct chromatin behavior to facilitate transcription, DNA replication, and DNA repair. ![]()
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